@article{IR,
      author = {Budrewicz, Jacqueline and Chavez, Shawn L.},
      url = {http://digitalcollections.ohsu.edu/record/9521},
      title = {Investigating the formation and rupture of micronucleir  and the consequence of cytosolic DNA release in early  mammalian embryogenesis},
      publisher = {Oregon Health and Science University},
      abstract = {The loss and/or gain of whole chromosomes, or aneuploidy,  that arises during mammalian preimplantation development  can lead to embryo arrest, implantation failure, or  spontaneous miscarriage in natural conception and assisted  reproduction. Segregation errors can occur meiotically  during gametogenesis, or post-zygotically from mitotic  cleavage divisions, resulting in the formation of abnormal  nuclear structures known as micronuclei that are spatially  isolated from the main nucleus. Micronuclei have been  detected in embryos from various mammals and are likely a  precursor to a dynamic process called cellular  fragmentation (CF), or cytoplasmic blebbing. Once formed, a  micronucleus may either fuse back with the primary nucleus  or persist and undergo unilateral inheritance in subsequent  mitotic divisions. Recent studies have shown that the  nuclear envelope of micronuclei in somatic cells is quite  fragile, and disruptions may cause genetic material to be  released into the cytoplasm, triggering the activation of  the cyclic GMP-AMP (cGAS)-cyclic GMP-AMP receptor  stimulator of interferon genes (STING) DNA-sensing pathway.  Using a non-human primate model and a combination of  time-lapse imaging, embryo immunostaining, and single-cell  DNA sequencing (scDNA-seq), we sought to determine whether  the cGAS-STING pathway is functional during preimplantation  development and if there are differences in micronuclei  fate between young and aged rhesus macaque females.},
      number = {IR},
      doi = {https://doi.org/10.6083/br86b414d},
      recid = {9521},
      address = {2022},
}