@article{IR,
      author = {Garrison, Zach},
      url = {http://digitalcollections.ohsu.edu/record/9532},
      title = {Adapting cyclic immunofluorescence for human skin and  melanoma},
      publisher = {Oregon Health and Science University},
      abstract = {One of the most important tools for diagnosis, analysis,  and study of cancer tissues is immunolabeling.  Simultaneously analyzing both expression and spatial data  of key surface markers provides important context when  understanding the state of cancer tissue. While the  standard approaches for labeling do allow for multiple  proteins to be identified simultaneously, the number of  markers is limited by spectral restrictions. Cyclic  immunofluorescence has been developed as an alternative to  standard multiplexed imaging strategies to allow for stains  of large number of markers on the same section of tissue.  Using a system of single stranded oligonucleotides, primary  antibodies are labeled and then conjugated to a fluorophore  via complementary imaging and docking strands which can  later be cleaved to allow for additional markers to be  imaged. This approach, developed by the Summer Gibbs lab,  was first validated against human breast cancer tissue. In  an attempt to expand its applicability to other tissue  types, I have worked on adapting the protocol and marker  panels for immune cells found in human skin tissue.},
      number = {IR},
      doi = {https://doi.org/10.6083/7p88ch16f},
      recid = {9532},
      address = {2022},
}