TY  - GEN
N2  - One of the most important tools for diagnosis, analysis, and study of cancer tissues is immunolabeling. Simultaneously analyzing both expression and spatial data of key surface markers provides important context when understanding the state of cancer tissue. While the standard approaches for labeling do allow for multiple proteins to be identified simultaneously, the number of markers is limited by spectral restrictions. Cyclic immunofluorescence has been developed as an alternative to standard multiplexed imaging strategies to allow for stains of large number of markers on the same section of tissue. Using a system of single stranded oligonucleotides, primary antibodies are labeled and then conjugated to a fluorophore via complementary imaging and docking strands which can later be cleaved to allow for additional markers to be imaged. This approach, developed by the Summer Gibbs lab, was first validated against human breast cancer tissue. In an attempt to expand its applicability to other tissue types, I have worked on adapting the protocol and marker panels for immune cells found in human skin tissue.
DO  - 10.6083/7p88ch16f
DO  - DOI
AB  - One of the most important tools for diagnosis, analysis, and study of cancer tissues is immunolabeling. Simultaneously analyzing both expression and spatial data of key surface markers provides important context when understanding the state of cancer tissue. While the standard approaches for labeling do allow for multiple proteins to be identified simultaneously, the number of markers is limited by spectral restrictions. Cyclic immunofluorescence has been developed as an alternative to standard multiplexed imaging strategies to allow for stains of large number of markers on the same section of tissue. Using a system of single stranded oligonucleotides, primary antibodies are labeled and then conjugated to a fluorophore via complementary imaging and docking strands which can later be cleaved to allow for additional markers to be imaged. This approach, developed by the Summer Gibbs lab, was first validated against human breast cancer tissue. In an attempt to expand its applicability to other tissue types, I have worked on adapting the protocol and marker panels for immune cells found in human skin tissue.
AD  - Oregon Health and Science University
T1  - Adapting cyclic immunofluorescence for human skin and melanoma
DA  - 2022
AU  - Garrison, Zach
L1  - https://digitalcollections.ohsu.edu/record/9532/files/Garrison-Zach-OHSU-ResearchWeek-2022.pdf
PB  - Oregon Health and Science University
PY  - 2022
ID  - 9532
L4  - https://digitalcollections.ohsu.edu/record/9532/files/Garrison-Zach-OHSU-ResearchWeek-2022.pdf
KW  - Melanoma
KW  - Neoplasms
KW  - Biomarkers
KW  - cyclic immunofluorescence
KW  - staining
KW  - cancer
TI  - Adapting cyclic immunofluorescence for human skin and melanoma
Y1  - 2022
L2  - https://digitalcollections.ohsu.edu/record/9532/files/Garrison-Zach-OHSU-ResearchWeek-2022.pdf
LK  - https://digitalcollections.ohsu.edu/record/9532/files/Garrison-Zach-OHSU-ResearchWeek-2022.pdf
UR  - https://digitalcollections.ohsu.edu/record/9532/files/Garrison-Zach-OHSU-ResearchWeek-2022.pdf
ER  -