000009532 001__ 9532
000009532 005__ 20240125142326.0
000009532 0247_ $$2DOI$$a10.6083/7p88ch16f
000009532 037__ $$aIR
000009532 245__ $$aAdapting cyclic immunofluorescence for human skin and melanoma
000009532 260__ $$bOregon Health and Science University
000009532 269__ $$a2022
000009532 336__ $$aAbstract
000009532 520__ $$aOne of the most important tools for diagnosis, analysis, and study of cancer tissues is immunolabeling. Simultaneously analyzing both expression and spatial data of key surface markers provides important context when understanding the state of cancer tissue. While the standard approaches for labeling do allow for multiple proteins to be identified simultaneously, the number of markers is limited by spectral restrictions. Cyclic immunofluorescence has been developed as an alternative to standard multiplexed imaging strategies to allow for stains of large number of markers on the same section of tissue. Using a system of single stranded oligonucleotides, primary antibodies are labeled and then conjugated to a fluorophore via complementary imaging and docking strands which can later be cleaved to allow for additional markers to be imaged. This approach, developed by the Summer Gibbs lab, was first validated against human breast cancer tissue. In an attempt to expand its applicability to other tissue types, I have worked on adapting the protocol and marker panels for immune cells found in human skin tissue.
000009532 540__ $$fCC BY
000009532 542__ $$fIn copyright - single owner
000009532 650__ $$aMelanoma$$021989
000009532 650__ $$aNeoplasms$$022758
000009532 650__ $$aBiomarkers$$028395
000009532 6531_ $$acyclic immunofluorescence
000009532 6531_ $$astaining
000009532 6531_ $$acancer
000009532 691__ $$aSchool of Medicine$$041369
000009532 692__ $$aDepartment of Dermatology$$041406
000009532 7001_ $$aGarrison, Zach$$uOregon Health and Science University$$041354
000009532 711__ $$aResearch Week$$uOregon Health and Science University
000009532 711__ $$d2022
000009532 8564_ $$94fbf524f-a3bd-48f1-beff-8274c9ecea20$$s321825$$uhttps://digitalcollections.ohsu.edu/record/9532/files/Garrison-Zach-OHSU-ResearchWeek-2022.pdf
000009532 905__ $$a/rest/prod/7p/88/ch/16/7p88ch16f
000009532 980__ $$aResearch Week