TY - GEN AB - The oral microbiome engages in a diverse array of highly sophisticated ecological interactions that are crucial for maintaining symbiosis with the host. Streptococci and corynebacteria are among the most abundant oral commensals and their interactions are critical for normal biofilm development. We have previously revealed a specifically symbiotic interaction between Streptococcus sanguinis and Corynebacterium durum via the release of C. durum fatty acids associated with membrane vesicles (MVs), enhancing bacterial fitness. Subsequently, S. sanguinis fatty acid metabolism-related genes have been studied. Glycerol dehydrogenase (gldA) was firstly characterized its essential role during the interspecies interaction. In this study, we have further characterized glycerol kinase (glpK) as another key gene being involved in S. sanguinis and C. durum interaction. In contrast to gldA, we have discovered a unique, complementary role of glpK during the interaction, in particularly, in the absence of glucose as a carbohydrate source of energy. Overall, our findings have supported the emerging role of commensal Corynebacterium spp. as major drivers of oral biofilm ecology, potentially shaping symbiotic health-associated biofilm communities. AD - Oregon Health and Science University AU - Treerat, June DA - 2022-04-07 DO - 10.6083/6395w8048 DO - DOI ED - Kreth, Jens ED - Merritt, Justin ED - Collaborator ED - Collaborator ID - 9540 KW - Glycerol Kinase KW - Fatty Acids KW - membrane vesicles KW - oral microbiome KW - symbiotic interaction L1 - https://digitalcollections.ohsu.edu/record/9540/files/Treerat-June-OHSU-ResearchWeek-2022.pdf L2 - https://digitalcollections.ohsu.edu/record/9540/files/Treerat-June-OHSU-ResearchWeek-2022.pdf L4 - https://digitalcollections.ohsu.edu/record/9540/files/Treerat-June-OHSU-ResearchWeek-2022.pdf LK - https://digitalcollections.ohsu.edu/record/9540/files/Treerat-June-OHSU-ResearchWeek-2022.pdf N2 - The oral microbiome engages in a diverse array of highly sophisticated ecological interactions that are crucial for maintaining symbiosis with the host. Streptococci and corynebacteria are among the most abundant oral commensals and their interactions are critical for normal biofilm development. We have previously revealed a specifically symbiotic interaction between Streptococcus sanguinis and Corynebacterium durum via the release of C. durum fatty acids associated with membrane vesicles (MVs), enhancing bacterial fitness. Subsequently, S. sanguinis fatty acid metabolism-related genes have been studied. Glycerol dehydrogenase (gldA) was firstly characterized its essential role during the interspecies interaction. In this study, we have further characterized glycerol kinase (glpK) as another key gene being involved in S. sanguinis and C. durum interaction. In contrast to gldA, we have discovered a unique, complementary role of glpK during the interaction, in particularly, in the absence of glucose as a carbohydrate source of energy. Overall, our findings have supported the emerging role of commensal Corynebacterium spp. as major drivers of oral biofilm ecology, potentially shaping symbiotic health-associated biofilm communities. PB - Oregon Health and Science University PY - 2022-04-07 T1 - Glycerol kinase (GlpK) during the oral microbiome interspecies interaction TI - Glycerol kinase (GlpK) during the oral microbiome interspecies interaction UR - https://digitalcollections.ohsu.edu/record/9540/files/Treerat-June-OHSU-ResearchWeek-2022.pdf Y1 - 2022-04-07 ER -