000009540 001__ 9540
000009540 005__ 20240125142337.0
000009540 0247_ $$2DOI$$a10.6083/6395w8048
000009540 037__ $$aIR
000009540 245__ $$aGlycerol kinase (GlpK) during the oral microbiome interspecies interaction
000009540 260__ $$bOregon Health and Science University
000009540 269__ $$a2022-04-07
000009540 336__ $$aAbstract
000009540 520__ $$aThe oral microbiome engages in a diverse array of highly sophisticated ecological interactions that are crucial for maintaining symbiosis with the host. Streptococci and corynebacteria are among the most abundant oral commensals and their interactions are critical for normal biofilm development. We have previously revealed a specifically symbiotic interaction between Streptococcus sanguinis and Corynebacterium durum via the release of C. durum fatty acids associated with membrane vesicles (MVs), enhancing bacterial fitness. Subsequently, S. sanguinis fatty acid metabolism-related genes have been studied. Glycerol dehydrogenase (gldA) was firstly characterized its essential role during the interspecies interaction. In this study, we have further characterized glycerol kinase (glpK) as another key gene being involved in S. sanguinis and C. durum interaction. In contrast to gldA, we have discovered a unique, complementary role of glpK during the interaction, in particularly, in the absence of glucose as a carbohydrate source of energy. Overall, our findings have supported the emerging role of commensal Corynebacterium spp. as major drivers of oral biofilm ecology, potentially shaping symbiotic health-associated biofilm communities.
000009540 540__ $$fCC BY
000009540 650__ $$aGlycerol Kinase$$019625
000009540 650__ $$aFatty Acids$$018911
000009540 6531_ $$amembrane vesicles
000009540 6531_ $$aoral microbiome
000009540 6531_ $$asymbiotic interaction
000009540 691__ $$aSchool of Dentistry$$041368
000009540 692__ $$aDepartment of Restorative Dentistry$$041446
000009540 7001_ $$aTreerat, June$$uOregon Health and Science University$$041354
000009540 711__ $$aResearch Week$$uOregon Health and Science University
000009540 711__ $$d2022
000009540 7201_ $$aKreth, Jens$$eCollaborator$$7Personal$$uOregon Health and Science University
000009540 7201_ $$aMerritt, Justin$$eCollaborator$$7Personal$$uOregon Health and Science University
000009540 8564_ $$9e96cab2c-e284-4a3b-ac0b-433d90601645$$s189465$$uhttps://digitalcollections.ohsu.edu/record/9540/files/Treerat-June-OHSU-ResearchWeek-2022.pdf
000009540 905__ $$a/rest/prod/63/95/w8/04/6395w8048
000009540 980__ $$aResearch Week