TY  - GEN
N2  - Although it is the smallest segment of the nephron, the distal convoluted tubule (DCT) plays an outsized role in electrolyte homeostasis through regulation of several ions, including sodium. In the DCT, sodium balance is primarily determined by the regulation of the sodium-chloride cotransporter (NCC). When phosphorylated, NCC reabsorbs sodium away from the lumen of the nephron. Dephosphorylation of NCC inactivates this reabsorption resulting in increased sodium excretion. A similar effect is leveraged by a common class of anti-hypertensive medications called thiazides, which block NCC activity. Gaining a better understanding of the molecular mechanism by which NCC is regulated holds high clinical relevance to several diseases including hypertension, FHHt, and Gitelman syndrome. To investigate a novel mechanism that may regulate NCC activity, we implemented a cell-specific chemogenetic approach with Designer Receptors Exclusively Activated by Designer Drugs (DREADD) technology.
DO  - 10.6083/rj430538n
DO  - DOI
AB  - Although it is the smallest segment of the nephron, the distal convoluted tubule (DCT) plays an outsized role in electrolyte homeostasis through regulation of several ions, including sodium. In the DCT, sodium balance is primarily determined by the regulation of the sodium-chloride cotransporter (NCC). When phosphorylated, NCC reabsorbs sodium away from the lumen of the nephron. Dephosphorylation of NCC inactivates this reabsorption resulting in increased sodium excretion. A similar effect is leveraged by a common class of anti-hypertensive medications called thiazides, which block NCC activity. Gaining a better understanding of the molecular mechanism by which NCC is regulated holds high clinical relevance to several diseases including hypertension, FHHt, and Gitelman syndrome. To investigate a novel mechanism that may regulate NCC activity, we implemented a cell-specific chemogenetic approach with Designer Receptors Exclusively Activated by Designer Drugs (DREADD) technology.
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
AD  - Oregon Health and Science University
T1  - Utilizing chemogenetic tools to study distal convoluted tubule function
DA  - 2022-04-08
AU  - Bradford, Tanner
AU  - Martz, Kevin
AU  - Su, Xiao-Tong
AU  - Maeoka, Yujiro
AU  - Sharma, Avika
AU  - McCormick, Jim
AU  - Ellison, David
AU  - Nelson, Jonathan
L1  - https://digitalcollections.ohsu.edu/record/9559/files/Bradford_Tanner_ResearchWeek2022_DREADDAbstract_OHSU_Format.pdf
PB  - Oregon Health and Science University
PY  - 2022-04-08
ID  - 9559
L4  - https://digitalcollections.ohsu.edu/record/9559/files/Bradford_Tanner_ResearchWeek2022_DREADDAbstract_OHSU_Format.pdf
KW  - Natriuresis
KW  - Kidney
KW  - Hypertension
KW  - DREADD
KW  - sodium-chloride cotransporter
KW  - distal convoluted tubule
KW  - chemogenetics
TI  - Utilizing chemogenetic tools to study distal convoluted tubule function
Y1  - 2022-04-08
L2  - https://digitalcollections.ohsu.edu/record/9559/files/Bradford_Tanner_ResearchWeek2022_DREADDAbstract_OHSU_Format.pdf
LK  - https://digitalcollections.ohsu.edu/record/9559/files/Bradford_Tanner_ResearchWeek2022_DREADDAbstract_OHSU_Format.pdf
UR  - https://digitalcollections.ohsu.edu/record/9559/files/Bradford_Tanner_ResearchWeek2022_DREADDAbstract_OHSU_Format.pdf
ER  -