TY  - THES
AB  - Enterohemorrhagic (EHEC) and enteropathogenic (EPEC) Escherichia coli strains pose significant health risks but are difficult to detect in environmental samples due to low abundance and reliance on time-consuming culture methods. This study evaluated the Sharp Laboratories of America (SLA) impedimetric biosensor platform for rapid, real-time detection of E. coli virulence genes—stx (EHEC), hly (EPEC), and adk (universal E. coli marker)—using DNA-DNA hybridization. The SLA system measures impedance changes during nucleic acid binding, enabling sensitive and specific detection of single-stranded versus double-stranded DNA. Successive platform iterations improved electrode design, assay throughput, and signal resolution. In collaboration with the Center for Coastal Margin Observation and Prediction (CMOP), this research optimized hybridization assays for pathogenic and non-pathogenic E. coli strains, supporting development of field-based biosensors for environmental monitoring. These findings demonstrate the potential of impedimetric detection for high-resolution, near real-time pathogen surveillance in aquatic ecosystems.
AD  - Oregon Health and Science University
AU  - Haynes, Vena
DA  - 2013
DO  - 10.6083/M47M05ZT
DO  - DOI
ED  - Simon, Holly
ED  - Advisor
ID  - 978
KW  - Biosensing Techniques
KW  - Biological Assay
KW  - Escherichia coli
KW  - Environmental Monitoring
KW  - Nucleic Acid Hybridization
KW  - Virulence
KW  - Enteropathogenic Escherichia coli
KW  - biosensors
L1  - https://digitalcollections.ohsu.edu/record/978/files/981_etd.pdf
L2  - https://digitalcollections.ohsu.edu/record/978/files/981_etd.pdf
L4  - https://digitalcollections.ohsu.edu/record/978/files/981_etd.pdf
LK  - https://digitalcollections.ohsu.edu/record/978/files/981_etd.pdf
N2  - Enterohemorrhagic (EHEC) and enteropathogenic (EPEC) Escherichia coli strains pose significant health risks but are difficult to detect in environmental samples due to low abundance and reliance on time-consuming culture methods. This study evaluated the Sharp Laboratories of America (SLA) impedimetric biosensor platform for rapid, real-time detection of E. coli virulence genes—stx (EHEC), hly (EPEC), and adk (universal E. coli marker)—using DNA-DNA hybridization. The SLA system measures impedance changes during nucleic acid binding, enabling sensitive and specific detection of single-stranded versus double-stranded DNA. Successive platform iterations improved electrode design, assay throughput, and signal resolution. In collaboration with the Center for Coastal Margin Observation and Prediction (CMOP), this research optimized hybridization assays for pathogenic and non-pathogenic E. coli strains, supporting development of field-based biosensors for environmental monitoring. These findings demonstrate the potential of impedimetric detection for high-resolution, near real-time pathogen surveillance in aquatic ecosystems.
PB  - Oregon Health and Science University
PY  - 2013
T1  - Development of novel impedimetric assays for rapid detection of E. coli
TI  - Development of novel impedimetric assays for rapid detection of E. coli
UR  - https://digitalcollections.ohsu.edu/record/978/files/981_etd.pdf
Y1  - 2013
ER  -