000000978 001__ 978
000000978 005__ 20260120144841.0
000000978 0247_ $$2DOI$$a10.6083/M47M05ZT
000000978 037__ $$aETD
000000978 245__ $$aDevelopment of novel impedimetric assays for rapid detection of E. coli
000000978 260__ $$bOregon Health and Science University
000000978 269__ $$a2013
000000978 336__ $$aThesis
000000978 502__ $$bM.S.
000000978 520__ $$aEnterohemorrhagic (EHEC) and enteropathogenic (EPEC) Escherichia coli strains pose significant health risks but are difficult to detect in environmental samples due to low abundance and reliance on time-consuming culture methods. This study evaluated the Sharp Laboratories of America (SLA) impedimetric biosensor platform for rapid, real-time detection of E. coli virulence genes—stx (EHEC), hly (EPEC), and adk (universal E. coli marker)—using DNA-DNA hybridization. The SLA system measures impedance changes during nucleic acid binding, enabling sensitive and specific detection of single-stranded versus double-stranded DNA. Successive platform iterations improved electrode design, assay throughput, and signal resolution. In collaboration with the Center for Coastal Margin Observation and Prediction (CMOP), this research optimized hybridization assays for pathogenic and non-pathogenic E. coli strains, supporting development of field-based biosensors for environmental monitoring. These findings demonstrate the potential of impedimetric detection for high-resolution, near real-time pathogen surveillance in aquatic ecosystems.
000000978 540__ $$fCC BY
000000978 542__ $$fIn copyright - single owner
000000978 650__ $$aBiosensing Techniques$$028362
000000978 650__ $$aBiological Assay$$015558
000000978 650__ $$aEscherichia coli$$018624
000000978 650__ $$aEnvironmental Monitoring$$018493
000000978 650__ $$aNucleic Acid Hybridization$$023046
000000978 650__ $$aVirulence$$027844
000000978 650__ $$aEnteropathogenic Escherichia coli$$037747
000000978 6531_ $$abiosensors
000000978 691__ $$aSchool of Medicine$$041369
000000978 692__ $$aDepartment of Environmental and Biomolecular Systems$$041414
000000978 7001_ $$aHaynes, Vena$$uOregon Health and Science University$$041354
000000978 7201_ $$aSimon, Holly$$uOregon Health and Science University$$041354$$7Personal$$eAdvisor
000000978 8564_ $$9eb2182a9-72b8-4094-88c7-9282eff2fe25$$s936247$$uhttps://digitalcollections.ohsu.edu/record/978/files/981_etd.pdf$$ePublic$$2c77eb8cac9e5e8f3ee1a682df3e6865e$$31
000000978 905__ $$a/rest/prod/r7/81/wg/01/r781wg01v
000000978 909CO $$ooai:digitalcollections.ohsu.edu:978$$pstudent-work
000000978 956__ $$aGet Accessible Copy$$uhttps://ohsu.libwizard.com/f/requestaccessibledocument
000000978 980__ $$aTheses and Dissertations