Cephalometric have been extensively used in the field of orthodontics since the introduction of the technique by Broadbent and Hoffrath in 1931. Many studies have been done to improve the technique and its application to clinical and research dentistry. The purpose of this study is to verify the reliability, validity, and feasibility of the three-dimensional cephalometric technique in an in vitro tooth movement study. The results should facilitate improved experimental designs in human applications.