Proteomic analysis of human saliva has been advanced by two-dimensional liquid chromatography (2-DLC), yet the parotid gland proteome remains incompletely characterized. This study applied 2-DLC coupled with tandem mass spectrometry to analyze parotid saliva collected under unstimulated and stimulated conditions from healthy adult males. Forty-nine parotid proteins were identified, representing a three- to fourfold increase over prior two-dimensional electrophoresis studies. No significant differences in protein abundance were observed between flow states. These findings establish a baseline parotid proteome and demonstrate that stimulated saliva can reliably be used for future biomarker, aging, and gland dysfunction studies.
