Microarray gene expression research frequency generates large numbers of questions or information needs throughout the course of the experiment. Many of these questions relate to the nature of the sequences on the micro array. To effectively analyze experimental data, it is necessary to maintain significant information about each of the 12,000 or more gene transcripts represented in the experiment. This thesis describes the development of a knowledge resource of DNA sequence functional annotations intended to aid researchers conducting microarray experiments. The resource includes an integrated data warehouse of sequence annotations for human and mice and data access tools for retrieving and utilizing the available information.