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Abstract
In these experiments, I begin to delineate the role of AML-derived exosomes in the regulation of the marrow microenvironment. Through electron and fluorescence microscopy, nanoparticle tracking analysis, and microRNA microarray, I demonstrate that AML blasts secrete exosomes containing concentrated microRNA, the makeup of which clearly distinguishes cells from exosomes and nonmalignant hematopoietic cells from AML blasts. These exosomes are taken up by both HSPC and marrow stroma, transferring sufficient microRNA to effect as much as a 10-fold increase in cellular concentration.