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Abstract
Cartwheel cells in the dorsal cochlear nucleus are glycinergic interneurons whose complex spike activity influences principal neuron responses. Using gramicidin perforated‑patch recordings in mouse brain slices, this study examined the ionic basis of complex spikes and regulation of the glycine reversal potential (Egly_\text{gly}gly). Complex spikes required both sodium and calcium currents, with specific calcium channel types shaping the slow depolarization and calcium‑activated potassium channels regulating repolarization. Complex spiking also produced calcium‑dependent intracellular acidification, leading to a negative shift in Egly_\text{gly}gly through activation of the Na+^++-driven Cl−^-−/HCO3−_3^-3− exchanger. This shift likely provides feedback inhibition to limit excessive spiking.