Anal cancer (AC) is one of the most common cancers among people living with Human Immunodeficiency Virus (HIV) and carries substantial morbidity and mortality. Current evidence supports screening people living with HIV (PLWH) with anal cytology or Papanicolaou smear (anal Pap) annually and treating precursors with ablation through high resolution anoscopy. However, there are no national consensus guidelines for AC screening among PLWH, which has led to large gaps in screening practices. This QI project aimed to improve provider’s knowledge, comfort, and willingness to screen for AC with anal Paps for cisgendered women living with HIV (WLWH). The methods, results and process were discussed in a final paper and presentation.