Microcosms in dentistry mimic the original environment's complexity, allowing variable manipulation and bacterial dynamics replication, diverging from single-species inoculation such as Streptococcus mutans. Therefore, the aim of this study was to compare bacterial activity between a hydrophilic and a hydrophobic material in the presence or absence of acquired salivary pellicle using a microcosm biofilm model from an inoculum derived from saliva. Open the Styles Pane to get easy access to all the styles defined in this template for formatting the text of your abstract.