Ischemic stroke is the most common type of stroke, accounting for about 87% of all stroke cases. Stroke is a leading cause of long-term disability and the second leading cause of death globally. Understanding the pathophysiology of ischemic stroke can improve early diagnosis and treatment, reduce neuronal damage, and improve recovery. Reactive astrogliosis and microglia are key features of stroke pathophysiology and may contribute to both neuroprotection and neurodysfunction. Here, we map the spatiotemporal progression of reactive astrogliosis through GFAP and vimentin labeling, and reactive microglia through Iba1 labeling. We found that reactive astrogliosis progresses faster and is more widespread than reactive microgliosis and that the astrocyte response is not entirely dependent on microglia. This response appears exacerbated in aging animals. This work also details preliminary findings linking reactive astrogliosis to impaired neurovascular coupling mechanisms after stroke and how this relates to developmental neurovascular coupling mechanisms.