Germin, a protein found in germinating wheat, has been identified as an oxalate oxidase (oxalate: oxygen oxidoreductase, EC 1.2.3.4) catalyzing the conversion of oxalate to carbon dioxide and hydrogen peroxide. Wheat germin is a homohexameric glycoprotein (~150 kDa) composed of 26 kDa subunits, each containing two potential N-glycan glycosylation sites. Wheat germin was mutated within its two potential NXS glycosylation sites (N47A, S49A, and N52A), and the recombinant enzyme was produced in a Pichia pastoris yeast expression system. One of these mutational variants (S49A) was prepared by high-density fermentation yielding 160 mg of purified oxalate oxidase.