Advancements in ion mobility technology, particularly Structures for Lossless Ion Manipulations (SLIM), have enabled the detailed characterization of heterogeneity of biomolecular structures in a sample. SLIM enables the resolution of subtle variations of molecular structures (e.g. separation of conformations, isotopes, double bond positions, stereoisomers, isotopologues, isotopomers, etc.) which could be markers for diseases. SLIM also enables IMS separations with high resolution, high throughput, high ion utilization efficiency. In this work, we will present SLIM technology and its game-changing ability to perform analytical separations with unprecedented utility and precision.