The clinical standard for small diameter vessel treatment, currently, is the use of autologous vessels. However, due to limited availability and pre-surgical complications, many patients are ineligible for this treatment. In addition, synthetic vascular grafts have been shown to exhibit limited long-term patency at diameters less than 6 mm, creating a large unmet clinical need. By biochemically modifying the surface of small diameter poly(vinyl alcohol) (PVA) grafts, anti-thrombotic properties can be achieved. Previously, thrombogenicity has been investigated through an ex vivo, non-human primate, shunt model. However, this model only characterizes platelet attachment over the length of the entire graft, losing the ability for dynamic physical properties of the thrombus along the graft to be understood. This work introduces a novel method to quantify thrombus formation along the cross-sectional area of biochemically-modified PVA grafts.