Bone tissue is a common site of metastasis, affecting a large number of patients with advanced cancer. The specificity of bone for extravasation of many types of cancers appears to be tightly related to the specific chemo-attractant molecules secreted by bone stromal cells, as well as possible interactions with other non-cancer cells, such as platelets, leukocytes, and monocytes/macrophages. In this study, we combined our rapid bone fabrication method with microfluidic systems to create an organ-on-a-chip system that can mimic the nanoscale mineralization of the native bone tissue and aspects that are relevant to cancer metastasis.