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Abstract
Light scattering and absorption govern optical imaging in tissues, yet current methods measure only reduced scattering, limiting structural insight. This thesis introduces a reflectance-mode confocal scanning laser microscopy (rCSLM) technique to simultaneously estimate scattering coefficient (μs) and anisotropy (g). A theoretical model mapping rCSLM-derived parameters to μs and g was validated using microsphere suspensions and applied to mouse tissues. The method revealed that optical clearing with glycerin increases g without reducing μs and detected structural changes in osteogenesis imperfecta dermis. These findings demonstrate rCSLM’s potential for non-invasive assessment of tissue architecture and pathology through optical properties.