The primary objective of this pilot study was to evaluate if there was a relationship between autonomic nervous system (ANS) activity during sleep, as quantified by a time domain measure of heart rate variability (SDNN), and masticatory muscle activity, as measured by the percent of the time a given muscle was active (duty factor). A secondary objective of this investigation was to analyze if anxiety (GAD-7), depression (PHQ-9), and physical symptoms (PHQ-15) scores, were correlated with ANS and masticatory muscle activity during sleep.