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Abstract
The zebrafish neuromuscular junction offers a unique system for studying synaptic transmission using electrophysiology and optical techniques. This work investigates two modes of vesicle release—synchronous and asynchronous—long recognized in classic neuromuscular models. Using paired recordings and pharmacological isolation, we characterized asynchronous release and its dependence on calcium dynamics. Optical imaging revealed that delayed, persistent asynchronous transmission is linked to calcium signals in motor neurons and sensitivity to the slow chelator EGTA. These findings provide mechanistic insight into asynchronous release and highlight the zebrafish neuromuscular junction as a powerful model for synaptic physiology.