This thesis investigates the role of Rabconnectin3 in auditory function using zebrafish as a genetic model for congenital deafness. Rabconnectin3 was identified as essential for synaptic vesicle acidification and proper hair cell function, critical for hearing and balance. Experimental approaches included developing a novel hearing assay, cloning and characterizing rabconnectin3 mutants, and analyzing its tissue and subcellular distribution. Findings reveal Rabconnectin3’s contribution to Vacuolar-type Proton Pump stability and vesicle lumen acidification, highlighting its previously unknown role in auditory physiology. These results advance understanding of genetic mechanisms underlying hearing loss.
