Cells in vivo are continuously exposed to a range of small messenger proteins (microenvironmental ligands). Microenvironmental ligands bind to cell-surface receptors and trigger protein cascades that impact many aspects of cell behavior. Aberrations in the ligand signaling network can drive the progression of many different pathologies, including cancer. Our prior research has characterized the molecular and phenotypic changes that occur in MCF10A cells, a mammary epithelial cell line, treated with a panel of six microenvironmental ligands that target different canonical pathways. Cells exposed to the perturbagens were assayed for gross phenotypic, proteomic, RNA expression, and epigenetic changes.