The efficacy of any antibacterial coating depends on their ability to directly contact the microorganisms they seek to eliminate. In the oral cavity, all surfaces are almost immediately coated with a layer of proteins known as the acquired salivary pellicle (ASP) once in contact with saliva. In this study, the antibacterial efficacy of a quaternary ammonium methacrylate (QAM) was tested in the presence of ASP, in terms of surface charge density and overall S. mutans elimination.