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Abstract

The bone tissue is a complex nanocomposite, organized in a calcified extracellular matrix. Bone autografts remain the gold standard to treat traumatic bone injuries. However, autografts can increase morbidity of the patient?s donor site and has limited availability. On the other hand, bioprinted microgels are attractive biomaterials that offer injectability, tunability, and acts as a cargo to deliver therapeutic proteins and cells. Here, we aim to bioprint and mineralize cell-laden gelatin methacrylate (GelMA) microgels and test the effects of cryopreservation on cell viability for bone tissue engineering applications.

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