Background: Spina bifida is a congenital neural tube defect affecting the spine, spinal cord, and brain with lifelong health-related consequences. Early recognition and comprehensive care improve long-term health, well-being, quality of life, and decrease the use of emergency services and hospitalizations. Current evidence-based care guidelines are not easily interpreted in busy care settings. Conclusions: The implementation of the intervention is dependent on provider buy-in and perceived benefit. Additional PDSA cycles are needed for continued modification of the schedule to support consistency and documentation of evidence-based guidelines in the care of patients with spina bifida. The periodicity schedule can be used in any care setting, takes minimal additional charting time, and would be helpful for tracking care metrics.