Urban watersheds experience anthropogenic impacts that degrade water quality. This study used fluorescence spectroscopy to characterize dissolved organic carbon (DOC) and [...]

This thesis work highlights technique development and emerging applications for improving super-resolution microscopy (SRM) throughput, analysis and applications in study [...]

Ubiquitin is a small 8 kDa protein that is appended onto lysine residues of proteins as a post-translational modification, and functions in regulating diverse cellular pr [...]

Light scattering and absorption govern optical imaging in tissues, yet current methods measure only reduced scattering, limiting structural insight. This thesis introduce [...]

This dissertation investigates the dynamics of intracellular signaling cascades as a functional readout of neuromodulation. Specifically, newly developed fluorescence lif [...]

Biological imaging relies on the ability to generate contrast between the target of interest and the cell. Proteins are specifically labeled with reporters compatible wit [...]

The goal of my PhD has been to develop and adapt optical chemical biology approaches to study signal transduction in cells. I developed genetically encoded FRET sensors t [...]

Over 300 million surgeries are performed worldwide each year. The ultimate goal of surgery is to repair damage or remove diseased tissues, while mi [...]

This dissertation describes my work on two main projects, first to expand and calibrate a site-directed fluorescence labeling method that can assess distance relationshi [...]

T-cell large granular lymphocytic leukemia (T-LGLL) is a rare chronic lymphoproliferative disorder of mature cytotoxic T-cells. Accurate diagnosis relies on the integrati [...]