The transcription factor MYC has been studied for decades as one of the most potent cancer drivers. Recent advances in next generation sequencing and molecular biology have uncovered a broad impact of MYC and its partners on the genome under both physiological and cancerous settings. This thesis investigates the interaction between MYC and PIN1, a previously identified regulator of MYC, with focus on the biophysical features, subnuclear localization, as well as the functional consequences. Such understanding potentiates more clinical utility of MYC by providing new biomarkers of MYC activity and novel strategies to inhibit MYC oncogenic functions.