While it is becoming more appreciated that non-hematopoietic cells within barrier tissues participate in and modulate the host immune response, their roles are still incompletely understood. Understanding how cell subsets within the tissue microenvironment regulate the immune response will provide more knowledge in designing tissue specific vaccination strategies as well as uncovering how inflammatory processes are dysregulated during chronic disease. Herein, I describe my thesis work where I investigate how two types of non-hematopoietic cells, lymphatic endothelial cells and tuft cells, influence downstream inflammatory responses in skin and small intestine. I demonstrate that lymphatic vessels, in response to viral cutaneous infection, remodel their cellular junctions.