White spot lesions are an unfortunate but common side effect of orthodontic treatment. Since treatment of white spot lesions after their formation is not guaranteed to be successful and is often invasive, being able to prevent their formation would be preferable. This study aimed to evaluate the effectiveness of orthodontic cements with an antimicrobial monomer (C14-MAM) and an ion releasing bioactive glass (BAG) on biofilm and white spot lesion (WSL) formation.