Mycobacterium tuberculosis (Mtb) remains a significant global health threat, where an estimated 10 million individuals suffered infection and around 1.4 million people died in 2019 alone. Its success as a pathogen is in part due to its ability to escape cellular immune responses via several mechanisms, among which is the manipulation of lipid metabolic pathways in the host. Previous studies have demonstrated that membrane sphingolipids are implicated in the first step of Mtb phagocytosis by different types of phagocytic cells. This study will shed light on the exact role of DAG during phagocytosis, such as determining whether it is critical at the pathogen contact site, phagocytic cup formation, or cellular entry. Understanding sphingolipid dynamics during Mtb infection would allow us to use novel approaches to combat this globally relevant bacterium.